cond2rep2_S5_L001_R2_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep2_S5_L001_R2_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1841075
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG	3395	0.18440313403853728	Illumina RNA PCR Primer (100% over 50bp)
AATGACGCTCGAACAGGCATGCCCCATGGAATACCAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACTG	3178	0.17261654196597098	Search with Blastn,more detail First hit on +100: Phoma sp. BPS96 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3100	0.0	8.702793	47
CGCAA	3245	0.0	7.8763423	42
CCGTA	3590	0.0	7.712708	46
GACGC	3560	0.0	7.6781974	4
GTGCG	3670	0.0	7.6413097	48
GGAAA	18265	0.0	7.132234	1
GCGCA	5035	0.0	7.120798	41
GCGTT	4155	0.0	6.920233	50
GTCGC	4425	0.0	6.899092	42
ATTCG	3710	0.0	6.889145	60
TGCGT	4185	0.0	6.7858033	49
CGAAC	3695	0.0	6.6288986	10
GGAGA	19010	0.0	6.4033637	1
CGCCG	4425	0.0	6.257316	44
TCGCG	1415	1.4842954E-9	6.020902	12
GGAAG	25040	0.0	5.970066	1
ACGCT	5105	0.0	5.8410416	5
TGACG	4690	0.0	5.8293347	3
GGCGC	5050	0.0	5.482896	40
GGACA	16010	0.0	5.3356104	1

No bad tiles