FastQCFastQC Report
Wed 21 Sep 2016
cond2rep2_S5_L004_R2_001.fastq.bz2

Summary

[OK]Basic Statistics

MeasureValue
Filenamecond2rep2_S5_L004_R2_001.fastq.bz2
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847516
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG34220.18522167061070108Illumina RNA PCR Primer (100% over 50bp)
AATGACGCTCGAACAGGCATGCCCCATGGAATACCAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACTG32060.17353029689594027Search with Blastn,more detail
First hit on +100: Phoma sp. BPS96 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAT28050.08.86202847
CGCAA31700.08.7347542
GTGCG35750.08.24460848
ATTCG36750.07.72744660
GCGCA52900.07.38082841
GGCGC50850.07.050152340
CGAAC37350.07.032301410
TGCGT42400.06.868703449
CCGTA36700.06.867637646
GGAAA183950.06.75873761
GGAGA199350.06.6642721
GACGC38050.06.62612874
GCGTT43150.06.584163750
GTCGC42850.05.964812342
ACGCT52400.05.8940645
GGAAG255700.05.89023261
CGCCG45750.05.66430844
TGACG48950.05.6592243
TTCGA54000.05.258814361
CCTCT209400.05.24178461

[OK]Bad tiles

No bad tiles

Produced by FastQC (version 0.11.5)