cond2rep1_S4_L002_R2_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep1_S4_L002_R2_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1950508
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG	5618	0.28802752923853686	Illumina RNA PCR Primer (100% over 50bp)
AGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAA	2977	0.1526269054010545	Search with Blastn,more detail First hit on +100: Rhinebothroides cf. freitasi LRP3929 28S ribosomal RNA gene, partial sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
GGAGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC	2960	0.15175533758384996	Search with Blastn,more detail First hit on +100: TPA_exp: Mus musculus ribosomal DNA, complete repeating unit Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
TGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCA	2373	0.12166061354272836	Search with Blastn,more detail First hit on +100: Homo sapiens clone LA13_165F6 sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3430	12.625893	47
CCGTA	4135	10.644924	46
GTCGC	5165	8.659575	42
GGAGA	19185	7.4376864	1
GGAAA	19185	7.289304	1
TACCG	2605	6.813318	6
AACGC	4470	6.670649	17
ATACG	3005	6.6151533	36
CGATT	4365	6.5871434	61
CAACG	4740	6.440454	16
ATCGA	4630	6.4401307	60
GTATC	7180	6.328225	48
CGACA	5180	6.098972	56
CGCAA	3225	6.0538173	32
CGGTG	7740	6.0079556	37
TAGCG	3670	5.996834	26
GCCGT	7375	5.968374	45
CGCCG	7370	5.924258	44
TCGCC	8240	5.858792	43
TCGGT	8015	5.846107	36

No bad tiles