cond2rep2_S5_L002_R1_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep2_S5_L002_R1_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1829940
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	5160	0.2819764582445326	Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	3341	0.18257429205329134	TruSeq Adapter, Index 5 (100% over 63bp)
TGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTC	2460	0.13443063707006786	Search with Blastn,more detail First hit on +100: PREDICTED: Cavia porcellus collagen alpha-1(I) chain-like (LOC100717127), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3290	10.034815	45
TCGTA	3505	9.216705	44
CGTCC	6220	8.903414	22
CTAGG	7205	8.228199	50
TATAT	11195	7.395739	1
GACGT	7770	7.1730022	20
CCTAG	8700	6.9774895	49
TAGGA	9700	6.2947583	51
GGTCG	4105	6.2264667	14
CCCTA	9975	6.156807	48
ACTTA	9405	6.152493	55
GCCGT	5200	6.1441455	50
GTCCG	4060	6.120605	18
AATCG	4190	6.100154	10
AGACG	9395	6.083471	19
TCTCG	5670	6.073108	42
ATATT	12445	6.0188	2
TATTA	9865	5.9735613	3
CGATA	2930	5.936778	58
GTCGT	4390	5.8222427	15

No bad tiles