cond2rep2_S5_L003_R1_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep2_S5_L003_R1_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1862912
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TATATTAATTTCATCTGAAGACGTCCTCCACTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCC	5160	0.27698570839631714	Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG	3190	0.17123728871787824	TruSeq Adapter, Index 5 (100% over 63bp)
TGACGTGCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTC	2503	0.13435954033255462	Search with Blastn,more detail First hit on +100: PREDICTED: Cavia porcellus collagen alpha-1(I) chain-like (LOC100717127), mRNA Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3360	0.0	8.134205	45
CGAAC	4385	0.0	7.3660636	50
TCGTA	3370	0.0	7.2674637	44
GTCCG	4250	0.0	7.266172	18
CGTCC	6645	0.0	7.211309	22
GGTCG	4270	0.0	7.1492023	14
GTCGT	4875	0.0	6.9172897	15
CTAGG	7370	0.0	6.8870344	50
TCGGT	4665	0.0	6.8478546	12
TATAT	11095	0.0	6.7214894	1
GCGAA	4565	0.0	6.688462	37
CGGTC	4660	0.0	6.474531	13
AATCG	4745	0.0	6.4331827	10
CCTAG	8620	0.0	6.2177515	49
ATAGG	5195	0.0	6.2175527	31
GACGT	8170	0.0	6.212661	20
CCGAC	5370	0.0	6.1471295	20
TCGAA	5185	0.0	6.092632	49
CGCGA	1765	3.8653525E-12	6.036011	1
TAGGG	5430	0.0	5.8832593	32

No bad tiles