FastQCFastQC Report
Wed 21 Sep 2016
cond2rep2_S5_L003_R2_001.fastq.bz2

Summary

[OK]Basic Statistics

MeasureValue
Filenamecond2rep2_S5_L003_R2_001.fastq.bz2
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862912
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG33950.18224156589253812Illumina RNA PCR Primer (100% over 50bp)
AATGACGCTCGAACAGGCATGCCCCATGGAATACCAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACTG31590.16957322729146626Search with Blastn,more detail
First hit on +100: Phoma sp. BPS96 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAA33550.09.52138342
GTGCG36350.09.37609148
ATTCG39150.08.34188960
CGTAT30350.08.070683547
GACGC40000.07.7229674
GCGTT42800.07.63171850
CGAAC39000.07.55376610
TGCGT44600.07.48312449
GGAAA180500.07.1746581
GCGCA51700.07.071448341
TGACG49450.06.9655223
CCGTA35550.06.68972946
ACGCT54850.06.47311455
GGCGC51900.06.360295340
GGAGA193100.06.1537691
TTCGA55450.06.08210261
GGAAG250800.06.0572761
CGCCG45450.05.935282744
TCGAA55050.05.8029249
GTCGC43900.05.740422742

[OK]Bad tiles

No bad tiles

Produced by FastQC (version 0.11.5)