cond2rep3_S6_L001_R2_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep3_S6_L001_R2_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1941505
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG	12161	0.6263697492409239	Illumina RNA PCR Primer (100% over 50bp)
TGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCA	3209	0.16528414812220418	Search with Blastn,more detail First hit on +100: Homo sapiens clone LA13_165F6 sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
CGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCG	2227	0.11470482950082539	Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3905	23.36218	47
CCGTA	4355	20.703646	46
GTCGC	5830	15.526476	42
GCCGT	7715	12.054983	45
CGCCG	7530	11.926879	44
GTATC	8115	11.679493	48
CGGTG	7980	11.4767275	37
TCGCC	8435	11.236406	43
GGTCG	9155	10.895553	41
ATACG	3235	10.643849	36
TCGGT	8860	10.416958	36
CGTGT	9165	10.109026	26
GAACG	11450	9.889775	23
AACGT	9420	9.760007	24
TACGA	3490	9.662721	37
CGCAA	3390	9.633616	32
ACGTG	10005	9.1538515	25
TATCA	11525	8.685792	49
TAGCG	3630	8.312154	26
CATTA	11685	8.202312	52

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