cond2rep3_S6_L004_R2_001.fastq.bz2 FastQC Report

Basic Statistics

Measure	Value
Filename	cond2rep3_S6_L004_R2_001.fastq.bz2
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1946380
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGGGGGGGGG	12213	0.6274725387642701	Illumina RNA PCR Primer (100% over 50bp)
TGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCA	3297	0.16939138297763026	Search with Blastn,more detail First hit on +100: Homo sapiens clone LA13_165F6 sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%
CGGATTGGAAGTTCTATTGGCAGAACGACTCGGTTATCAACTTCTAGCAGTCGTAGTTCACCAGGTTTTAGGTCG	2111	0.10845775234024188	Search with Blastn,more detail First hit on +100: Mus musculus mitochondrial DNA from Lewis lung carcinoma, complete sequence Evalue=3.3219e-33, Ident=100%, QueryCovergap=0%

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTAT	3730	24.555645	47
CCGTA	4440	20.78673	46
GTCGC	5475	16.272411	42
CGCCG	7135	12.786008	44
GTATC	7715	12.148414	48
TCGCC	8205	11.767251	43
CGGTG	7790	11.663866	37
GCCGT	8225	11.479991	45
TCGGT	8585	11.162552	36
GGTCG	9015	10.826694	41
AACGT	8980	10.2373085	24
CGTGT	9260	9.851094	26
GAACG	11155	9.768574	23
ACGTG	10175	9.139408	25
TATCA	11130	8.966188	49
TCTCG	11605	8.839015	34
ATACG	3175	8.831274	36
CATTA	11290	8.494198	52
CTCGG	12580	8.323029	35
GTGTA	12455	8.036307	27

No bad tiles